A simple effective procedure for the determination of adenosine triphosphate in soils
โ Scribed by Q.Y. Bai; L. Zelles; I. Scheunert; F. Korte
- Book ID
- 119022444
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 402 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0045-6535
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๐ SIMILAR VOLUMES
A simple method for preparing s-32P-labeled nucleoside-5'-triphosphates of high specific activity is presented. Unprotected nucleosides are heated dry with urea and inorganic phosphate. The products from such reactions are phosphorylated enzymatically.
Marsh (1) has proposed a procedure for the estimation of inorganic orthophosphate (Pi) in the presence of ATP, which is purportedly designed to overcome errors resulting from molybdate-catalyzed hydrolysis of ATP (2). In a critique of methods currently used for the assay of Pi in the presence of lab