## 435 1.5 to 15 pg of chymotrypsin. However, the present method is somewhat simpler with respect to the number of solutions which must be prepared, and, since the velocity is measured by the increase in absorbance at 385 mp, it can be easily modified for use with spectrophotometers not having the
A simple arrangement for the concentration of protein solutions
β Scribed by Bengt v. Hofsten; Sven-Olof Falkbring
- Publisher
- Elsevier Science
- Year
- 1960
- Tongue
- English
- Weight
- 210 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Construction of a simple centrifuge column from readily available components is described which may be used for removing salt from protein solutions.
Counting was performed by a Packard Tricarb 1600TR liquid scintillation counter. Although partial hydrolysis of P-containing organic compounds may have led to an overestimate of the tissue content of labeled phosphate, overcoming this problem is a complex procedure (8). However, any overestimate in
## Abstract We propose simple expressions $\Pi /\Pi \_0 = 1 + (\varphi /\varphi \_{{\rm ex}} )^{3\alpha - 1}$ and $(\delta \_0 /\delta )^2 = 1 + (\varphi /\varphi \_{{\rm ex}} )^{2\alpha }$ for the osmotic pressure Ξ and the depletion thickness __Ξ΄__ as a function of the polymer concentration __Ο__