We report below on an extremely simple and somewhat more sensitive modification of the Chert procedure (1) for inorganic orthophosphate determination that has been routinely used in this laboratory for almost 2 years. The method overcomes the problem of precipitation of the phosphomolybdate complex
A simple and sensitive apparatus for continuous monitoring of orthophosphate in the presence of acid-labile compounds
โ Scribed by A.A. Baykov; S.M. Avaeva
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 265 KB
- Volume
- 116
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
An inexpensive
analyzer for continuous recording of orthophosphate concentration has been designed utilizing instruments used in routine liquid chromatography.
The determination is based on the change in the spectrum of methyl green upon binding to molybdophosphoric acid (H. Van Belle, 1970, Anal. Biochem. 33, 132-142).
Changes in PI concentrations down to I and 5 pM in a sample can be displayed full-scale on the recorder at sample consumption rates of 7 and 1.4 ml/min, respectively. Short time of sample contact with acid (down to 6 s) enables measurements to be made in the presence of highly labile compounds such as acetyl phosphate.
๐ SIMILAR VOLUMES
A chemiluminescence (CL) determination of adenyl compounds is described. CL derivatization of adenyl compounds with methylglyoxal dimethyl acetal was performed in the presence of tungstosilicic acid and propan-2-ol. CL from adenyl compounds was produced by hydrogen peroxide and L-cysteine ethyl este