Chemiluminescent derivatization of adenyl compounds with glyoxal derivatives in the presence of heteropoly acids and its application to the simple and sensitive determination of DNA
✍ Scribed by Kuroda, Naotaka ;Nakashima, Kenichiro ;Akiyama, Shuzo ;Sato, Naofumi ;Imi, Noriko ;Shirakawa, Kamon ;Uemura, Akio
- Publisher
- John Wiley and Sons
- Year
- 1998
- Weight
- 201 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0884-3996
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✦ Synopsis
A chemiluminescence (CL) determination of adenyl compounds is described. CL derivatization of adenyl compounds with methylglyoxal dimethyl acetal was performed in the presence of tungstosilicic acid and propan-2-ol. CL from adenyl compounds was produced by hydrogen peroxide and L-cysteine ethyl ester in DMF and water. The proposed method is highly sensitive and speci®c to compounds containing adenine. Adenine was determined in the range 1.0 Â 10 À3 ±5.0 Â 10 À8 M with the detection limit of 3.0 Â 10 À8 M (150 fmol per assay). The method was applied to the determination of DNA and detection limits of a few nanograms of DNA achieved.