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A simple and reliable method for rapid production and purification of Pseudomonas aeruginosa haemolytic phospholipase C

โœ Scribed by A. Dubouix; M. Nieto; J. Fauvel; H. Chap; N. Marty; J.-P. Salles; F. Gaits


Book ID
111190339
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
95 KB
Volume
38
Category
Article
ISSN
0266-8254

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Profilin has been purified from bovine thyroid by a new and simple method, employing affinity chromatography on DNase I-agarose and gel filtration. This procedure should be particularly useful for purifying profilin from tissues in which it is present in relatively low concentration because it does