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A simple and rapid micro-Kjeldahl method for total nitrogen analysis

✍ Scribed by D. V. Guebel; B. C. Nudel; A. M. Giulietti

Publisher
Springer-Verlag
Year
1991
Tongue
English
Weight
277 KB
Volume
5
Category
Article
ISSN
0951-208X

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✦ Synopsis

A fast and simple micro-method for total nitrogen analysis in complex fermentation media is presented. Samples containing up to 50 ug nitrogen are digested in tubes with sodium selenite (in sulphuric-phosphoric mixture) plus hydrogen peroxide during one hour at 38O'C. Ammonium liberated is measured without extraction by Berthelot calorimetric reaction.

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## Abstract A rapid single step method for direct determination of total fatty matter (TFM) of soaps has been developed. The procedure involves two‐phase titration of a 2% solution of soap in ethanol : 2‐propanol (4 : 1 v/v) under alkaline conditions with 0.01 M solution of p‐tert. octyl phenoxyeth