A simple and rapid micro-Kjeldahl method
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D. V. Guebel; B. C. Nudel; A. M. Giulietti
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Article
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1991
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Springer-Verlag
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English
โ 277 KB
A fast and simple micro-method for total nitrogen analysis in complex fermentation media is presented. Samples containing up to 50 ug nitrogen are digested in tubes with sodium selenite (in sulphuric-phosphoric mixture) plus hydrogen peroxide during one hour at 38O'C. Ammonium liberated is measured