A simple and rapid assay for tyrosine aminotransferase
โ Scribed by Fiona A.O. Marston; Christopher I. Pogson
- Book ID
- 115908118
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 303 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0014-5793
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Tyrosine hydroxylase can be measured by release of tritiated water from labeled tyrosine, and the assay method has now been modified to allow recovery of 3H2O from the reaction mixture in a much more rapid and less tedious manner than previously possible. In the new method, the tyrosine hydroxylase
A simple, rapid, and sensitive fluorometric assay for measuring the activity of tyrosine hydroxylase is described. The enzyme activity is detected by converting tyrosine to 3,4-dihydroxyphenylalanine (dopa), which is then subjected to conversion to the highly fluorescent product by the trihydroxyind
## COMJIUNICATIONs muscle and kidney at pH 4.6 yields a filtrate containing all the tissue magnesium and calcium, with amounts of heavy metals too low to interfere with EDTA titration of these elements using eriochrome black T or Cal-Red as indicators.