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A Simple and Quantitative Purification of Glycosphingolipids and Phospholipids by Thin-Layer Chromatography Blotting

โœ Scribed by T. Taki; T. Kasama; S. Handa; D. Ishikawa

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
730 KB
Volume
223
Category
Article
ISSN
0003-2697

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๐Ÿ“œ SIMILAR VOLUMES

Direct Mass Spectrometric Analysis of Gl
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โœ T. Taki; D. Ishikawa; S. Handa; T. Kasama ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 267 KB

A simple, rapid method for the analysis of glycosphingolipid that combines "thin-layer chromatography (TLC) blotting" and mass spectrometry is reported. Glycosphingolipids developed by TLC were transferred to a polyvinylidene difluoride membrane by TLC blotting, after which the glycosphingolipid ban

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A simple method of blotting glycosphingolipids from a high-performance thin-layer chromatography (HPTLC) plate to a polyvinylidene difluoride (PVDF) membrane is described. The developed HPTLC plate is dipped in a solvent mixture (isopropanol/0.2\% \(\mathbf{C a C l}_{2}\) / methanol, \(40 / 20 / 7\)