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A simple and fast method for cloning and analyzing polymerase chain reaction products

โœ Scribed by Boris Skryabin; Dido Vassilacopoulou


Book ID
113409595
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
461 KB
Volume
10
Category
Article
ISSN
1050-3862

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A novel system for the detection of polymerase chain reaction (PCR) products has been developed. The system is based on a PCR in which one of the primers is biotinylated and digoxigenin-11-dUTP is incorporated during elongation. Biotinylated PCR products are captured on streptavidin-coated solid sup