A simple and efficient method to remove ribonuclease contamination from pancreatic deoxyribonuclease preparations
โ Scribed by Oliver Brison; Pierre Chambon
- Book ID
- 102629014
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 469 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
An affinity chromatography method which efficiently removes the contaminating RNase activity from commercial pancreatic DNase preparations is described. The new method is compared with the iodoacetate treatment previously used for the preferential inactivation of RNase.
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A rapid and efficient method is described for the removal of radio-active molecular probes from nucleic acids immobilized on nylon membranes. This method involves boiling in distilled water in a microwave oven. This procedure can be completed within ten minutes, does not require the use of any buffe