✦ LIBER ✦
A rapid and efficient method to remove labelled molecular probes from nucleic acids immobilized on solid supports
✍ Scribed by Charlene P. Wight; David T. Dennis; Reginald H. Lau
- Book ID
- 104768197
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 214 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0301-4851
No coin nor oath required. For personal study only.
✦ Synopsis
A rapid and efficient method is described for the removal of radio-active molecular probes from nucleic acids immobilized on nylon membranes. This method involves boiling in distilled water in a microwave oven. This procedure can be completed within ten minutes, does not require the use of any buffers or reagents, and produces results comparable with conventional buffer-wash procedures recommended by the suppliers of the transfer membranes.