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A simple and efficient method for obtaining transgenic soybean callus tissues

✍ Scribed by Dong Liu, Sheng Liu, Dasong Chang, Lu Wang, Dan Wang…

Book ID
120948766
Publisher
Springer-Verlag
Year
2013
Tongue
English
Weight
618 KB
Volume
35
Category
Article
ISSN
0137-5881

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Cotyledons from germinating seeds of the soybean cultivar Peking were inoculated with virulent Agrobacterium tumefaciens strain A281:pZA-7 which carries a wild type Ti plasmid pTiBo542 and a disarmed Ti plasmid (a binary vector)pZA-7 which contains the βglucuronidase (uidA) and neomycin phosphotrans