A simple activity staining protocol for lipases and esterases
β Scribed by Rajni Singh; Namita Gupta; Vineet Kumar Goswami; Rani Gupta
- Book ID
- 105950116
- Publisher
- Springer
- Year
- 2006
- Tongue
- English
- Weight
- 390 KB
- Volume
- 70
- Category
- Article
- ISSN
- 1432-0614
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract Longβchain fatty acid esters of 7β(3,4βdihydroxybutyloxy)β2__H__β1βbenzopyranβ2βone (**6**) such as octanoate **2a** are shown to be exceptionally sensitive and selective fluorogenic substrates for lipases and esterases. Umbelliferone (**8**) is released upon hydrolysis of the ester fun
## Abstract Fluorogenic cyanohydrin esters were prepared that release the fluorescent product umbelliferone by secondary decomposition of the primary cyanohydrin reaction product by cyanide elimination to the aldehyde and subsequent Ξ²βelimination. Whereas butyrate **1b** and octanoate **1d** show t