A set of aspartyl protease-deficient strains for improved expression of heterologous proteins in Kluyveromyces lactis
β Scribed by Mehul B. Ganatra; Saulius Vainauskas; Julia M. Hong; Troy E. Taylor; John-Paul M. Denson; Dominic Esposito; Jeremiah D. Read; Hana Schmeisser; Kathryn C. Zoon; James L. Hartley; Christopher H. Taron
- Book ID
- 109295766
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 516 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1567-1356
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A gratuitous induction system in the yeast Kluyveromyces lactis was evaluated for the expression of intracellular and extracellular products during fed-batch culture. The Escherichia coli lacZ gene (beta-galactosidase; intracellular) and MFalpha1 leader-BPTI cassette (bovine pancreatic trypsin inhib
## Abstract Because of its eukaryotic nature, simple fermentation requirements, and pliable genetics, there have been many attempts at improving recombinant protein production in __Saccharomyces__ __cerevisiae__. These strategies typically involve altering the expression of a native protein thought