Release of virus-like particles by osmot
✍
Pablo Álvarez; Miguel Sánchez; María Molina; César Nombela
📂
Article
📅
1995
🏛
Springer-Verlag
🌐
English
⚖ 376 KB
A yeast system based on a S. cerevisiae mutant strain deficient in cell integrity, was used to achieve the rclcasc of yeast expressed virus-like particles (vI.Ps) by simple osmotic shock. Yeast cells, grown in the prcscnce of IM sorbitol, lysed and rcleac;ed the intracellular content upon being tran