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A new system for the release of heterologous proteins from yeast based on mutant strains deficient in cell integrity

✍ Scribed by Pablo Alvarez; Marta Sampedro; María Molina; César Nombela


Book ID
118234489
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
621 KB
Volume
38
Category
Article
ISSN
0168-1656

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A yeast system based on a S. cerevisiae mutant strain deficient in cell integrity, was used to achieve the rclcasc of yeast expressed virus-like particles (vI.Ps) by simple osmotic shock. Yeast cells, grown in the prcscnce of IM sorbitol, lysed and rcleac;ed the intracellular content upon being tran