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A separation method for the assay of adenylylcyclase, intracellular cyclic AMP, and cyclic-AMP phosphodiesterase using tritium-labeled substrates

โœ Scribed by Robert Alvarez; Donald V. Daniels


Book ID
107719003
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
508 KB
Volume
203
Category
Article
ISSN
0003-2697

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A new method for separating cyclic AMP f
โœ Asru K. Sinha; Robert W. Colman ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 567 KB

A new method for assay of cyclic AMP phosphodiesterase (EC 3.1.4.17) has been developed based on the observation that a mixture of cyclic AMP and AMP can be resolved on a column of florisil (activated magnesium silicate) at pH 7.0. The cyclic nucleotide is retained by the silicate and the AMP which