Standardization of the assay for the catalytic subunit of cyclic AMP-dependent protein kinase using a synthetic peptide substrate
โ Scribed by Becky A. de la Houssaye; Ruthann A. Masaracchia
- Book ID
- 107711860
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 452 KB
- Volume
- 128
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
We investigated the effects of the purified catalytic subunit (C subunit) of the cAMP-dependent protein kinase (A-kinase) on the cardiac Na+ channel currents. Single Na+ channel currents in guinea-pig ventricular myocytes were recorded using the patch clamp technique of the inside-out configuration.
Currently, protein phosphatase 2B (calcineurin) activity is assayed based on release of [32P]phosphate from a 19-amino acid peptide (partial sequence of the regulatory subunit of cAMP-dependent protein kinase) following its [32P]ATP phosphorylation using the catalytic subunit of cAMP-dependent prote