A sensitive high-performance liquid chromatographic assay for the cholecystokinin-b antagonist, CI-988, in human and monkey plasma

✍ Scribed by Joanna P. Hinton; Kathleen Jennings; Elvin L. Johnson; D. Scott Wright

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 385 KB
• Volume: 9
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1130090208

✦ Synopsis

A sensitive and specific high-performance liquid chromatographic assay for the non-peptide cholecystokinin subtype B receptor antagonist, C1-988, in human and cynomolgus monkey plasma has been developed and validated. The method involves isolation of CI-988 and internal standard by batch robotic solid phase extraction with a C,, cartridge, liquid chromatographic separation on a CI8 column and quantitation by fluorescence detection. The human plasma assay is linear from 0.25 to 500ng/mL for a 1.00-mL plasma aliquot. Assay precision for C1-988 based on human plasma quality control samples was within +7.2% relative standard deviation with an accuracy of f5.6% relative error. The monkey plasma assay is linear from 1.00 to 250 ng/mL for a 0.500-mL plasma aliquot. Assay precision based on monkey plasma quality control samples was within i 11.0% relative standard deviation with an accuracy of *2.6% relative error.