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A sensitive fluorimetric microassay for the determination of glutathione peroxidase activity. Application to human blood platelets

✍ Scribed by Juan Ignacio Ramos Martinez; Jean-Marie Launay; Claude Dreux

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
346 KB
Volume
98
Category
Article
ISSN
0003-2697

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✦ Synopsis

The method proposed for measuring glutathione peroxydase (GSH-Px) activity is based on the determination of oxidized glutathione (GSSG) using o-phtalaldehyde (OPT) as a fluorescent reagent. This method makes it possible to study the kinetics of both substrates (peroxide and reduced ghrtathione, GSH), and allosteric kinetics were found for GSH. with human platelets as the source of GSH-Px. Different methods for platelet disruption were compared. The reference values obtained for GSH-Px activity in human blood platelets by this fluorimetric procedure and the conventional enzymatic method were very similar and significantly higher than those previously reported; the reasons for this difference are discussed.

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