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A sensitive fluorimetric assay for γ-glutamyl transferase

✍ Scribed by G.D. Smith; J.L. Ding; T.J. Peters

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
283 KB
Volume
100
Category
Article
ISSN
0003-2697

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✦ Synopsis

A rapid, sensitive single-step assay for y-glutamyl transferase is presented using L-Yglutamyl-7-amino-4-methyl-coumarin as the donor substrate. Kinetic parameters and the relative activities with different acceptors were determined using this substrate. The results are compared with those obtained with an alternative substrate, L-y-glutamyl-2-naphthylamide.

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