✦ LIBER ✦

A fluorimetric assay for cysteine sulfinate transaminase

✍ Scribed by James M. Schaeffer; Pierre Mallorga; Lawrence Austin; Paul Mandel

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 326 KB
Volume: 90
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90028-3

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✦ Synopsis

Cysteine sulfinate transaminase (E.C. 2.6.1, L-cysteine sulfinate:2 oxoglutarate aminotransferase) catalyzes the conversion of cysteine sulfinate and a-ketoglutarate to 3-sulfonyl pyruvate and glutamate. A simple two-step assay has been developed to measure the enzyme activity in the high speed supematant of whole brain homogenate. In the first step, the supematant is incubated in the presence of exogenous substrate, then glutamate dehydrogenase is added to catalyze the conversion of glutamate to a-ketoglutarate. and the concomitant production of NADH is fluorimetrically monitored. The apparent K, values of cysteine sulfinate transaminase for cysteine sulfinate and cY-ketoglutarate are 1.24 and 0.22 mtvr, respectively. This assay is extremely rapid and has a high sensitivity, samples containing as low as 30 ng of protein may be accurately assayed.

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