The ATTTT repeat polymorphism located approximately 1,400 base pairs (bp) upstream from the beta-globin structural gene was analyzed by denaturing gradient gel electrophoresis (DGGE) of RNA:DNA duplexes. A study of 81 unrelated Japanese from Hiroshima revealed a sequence heteromorphism in this site.
โฆ LIBER โฆ
A Sensitive Denaturing Gradient-Gel Electrophoresis Assay Reveals a High Frequency of Heteroplasmy in Hypervariable Region 1 of the Human mtDNA Control Region
โ Scribed by Lois A. Tully; Thomas J. Parsons; Robert J. Steighner; Mitchell M. Holland; Michael A. Marino; Valerie L. Prenger
- Book ID
- 117853366
- Publisher
- American Society of Human Genetics
- Year
- 2000
- Tongue
- English
- Weight
- 526 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0002-9297
- DOI
- 10.1086/302996
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Denaturing gradient gel electrophoresis (DGGE) was used in combination with the polymerase chain reaction (PCR) to detect sequence variations in the promoter region of the low density lipoprotein receptor (LDLR) gene. On the basis of calculated predictive melting properties we designed primers to am