A sensitive continuous-flow bioluminescent system for determining ethanol in serum and saliva

✍ Scribed by S. Girotti; A. Roda; S. Ghini; A.L. Piacentini; G. Carrea; R. Bovara

• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 665 KB
• Volume: 183
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(86)80086-1

✦ Synopsis

A continuous-flow system, based on NAD(P)H:FMN oxidoreductase and bacterial luciferase co-immobilized on a nylon coil placed in front of a photomultiplier, and alcohol dehydrogenase separately immobilized on a second nylon coil, is described for the assay of ethanol in serum and saliva. The flow is air-segmented and 5-504 samples are required. The sample throughput is 25-30 h-* with no carryover. The detection limit is 1 Mmol 1-l ethanol (50 pmol injected) and the response is linear between 50 and 2500 pmol of ethanol. The relative standard deviation is 3-10% for both within-day and between-day assays, and serum and saliva can be analyzed directly. The immobilized enzymes are satisfactorily stable and up to 900 samples can be analyzed with one enzyme reactor.