Collagenase is assayed by incubation with soluble, telopeptide-free collagen extracted from rat skin and labeled with [2-3H]acetic anhydride. Collagen is cleaved by collagenase and the resulting fragments are digested with trypsin and chymotrypsin. Undigested collagen is recovered by precipitation w
A sensitive collagenase assay using [3H]collagen labeled by reaction with pyridoxal phosphate and [3H]borohydride
✍ Scribed by Henning Birkedal-Hansen; Keld Danø
- Book ID
- 102983476
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 766 KB
- Volume
- 115
- Category
- Article
- ISSN
- 0003-2697
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