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A sensitive and inexpensive high-performance liquid chromatographic assay for tyrosine hydroxylase

โœ Scribed by T. Kato; S. Horiuchi; A. Togari; T. Nagatsu


Publisher
Springer
Year
1981
Tongue
English
Weight
335 KB
Volume
37
Category
Article
ISSN
1420-682X

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A simple and sensitive fluorometric assa
โœ Takashi Yamauchi; Hitoshi Fujisawa ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 419 KB

A simple, rapid, and sensitive fluorometric assay for measuring the activity of tyrosine hydroxylase is described. The enzyme activity is detected by converting tyrosine to 3,4-dihydroxyphenylalanine (dopa), which is then subjected to conversion to the highly fluorescent product by the trihydroxyind