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A Semicontinuous Prokaryotic Coupled Transcription/Translation System Using a Dialysis Membrane

✍ Scribed by Dong-Myung Kim; Cha-Yong Choi


Book ID
109387887
Publisher
American Institute of Chemical Engineers
Year
1996
Tongue
English
Weight
105 KB
Volume
12
Category
Article
ISSN
8756-7938

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A novel bioreactor using a hollow-fiber membrane was developed for the coupled transcription/ translation system using T7 RNA polymerase and Escherichia coli S30 extract. The large surface area per the reaction volume of the reactor assured rapid mass transfers of substrates into the reaction mixtur