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A semi-automated technique for the isolation of live lymphocyte suspensions from peripheral blood

✍ Scribed by Keith Gelsthorpe; Robert W. Doughty; Miles Fox

Publisher: John Wiley and Sons
Year: 1970
Tongue: English
Weight: 288 KB
Volume: 57
Category: Article
ISSN: 0007-1323
DOI: 10.1002/bjs.1800570511

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✦ Synopsis

Abstract

A semi-automated method has been devised for the separation of viable human peripheral blood lymphocytes from standard units of donor blood. The tech- nique is based on the carbonyl iron method (Levine, 1956; Cassen, Hitt, and Hays, 1958), but using a continuous-flow system. Lymphocytes harvested by this technique have been used in the production of heterologous antilymphocytic serum (ALS).

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