A semi-automated highly sensitive approach for mutation detection
✍ Scribed by Regine Dahse; Wolfgang Fiedler; Ulrike Riese; Ferdinand v. Eggeling; Günther Ernst
- Book ID
- 114393083
- Publisher
- Elsevier
- Year
- 1998
- Weight
- 123 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1366-2120
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
We have developed a rapid and highly sensitive method for the detection of mutant K-ras codon 12 allele in the presence of 10 5 copies of the wild-type alleles. This sensitivity is achieved by selective amplification of mutant K-ras sequences, using a two-stage procedure with modified primers. In th
## Detection of molecular features such as K-ras mutations has been used to evaluate potential tumour markers in a wide variety of clinical samples. Here we have applied a recently developed highly sensitive method for detection of K-ras codon 12 mutations to colorectal and pancreatic cancer diagno
## Abstract The catalytic action of nitroprusside on the phenol hypochlorite reaction has been utilised to design a more sensitive automated technique for determining ammonium nitrogen in soil extracts. The method gives reproducible results in close agreement with manual methods. Sensitivity is suc