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A search for restriction fragment length polymorphism on the human Y chromosome

✍ Scribed by Sibylle Jakubiczka; Joachim Arnemann; Howard J. Cooke; Michael Krawczak; Jörg Schmidtke

Book ID
104666196
Publisher
Springer
Year
1989
Tongue
English
Weight
243 KB
Volume
84
Category
Article
ISSN
0340-6717

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✦ Synopsis

A systematic search for restriction fragment length polymorphisms (RFLPs) on the human Y chromosome was performed. DNA samples from 16-34 individuals were screened with five restriction enzymes and 12 Y-chromosomal probes, 3 of which detect lowly repetitive sequences and 9 of which are apparently single copy in genomic DNA. None of the single-copy probes revealed any variation. The repetitive sequence probe p21A1 (DYZ?) revealed a TaqI RFLP with q = 0.05. The frequency of fixed point mutations in Y-chromosomal DNA outside the pseudoautosomal region is probably less than 1 in 18,000 bp.

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