The DNA binding protein B' preparation, isolated from the membrane of E. coli, recognizes two sites, one of which is located in the minimum oriC (35-270 bp) and the other between base pairs 417 and 488. Recognition is only possible when restriction fragments containing these sites are in single-stra
✦ LIBER ✦
A role for the weak DnaA binding sites in bacterial replication origins
✍ Scribed by Godefroid Charbon; Anders Løbner-Olesen
- Book ID
- 110837032
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 102 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0950-382X
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Scatchard analysis of binding of 125I-basic fibroblast growth factor (FGF) to baby hamster kidney (BHK) cells revealed the presence of two binding sites: a high affinity site with KD of 20 pM and 80,000 sites per cell and a low affinity site with KD of about 2 nM and 600,000 sites per cell. The bind