Recognition sites for a membrane-derived DNA binding protein preparation in the E. coli replication origin

✍ Scribed by Jacq, Annick ;Kohiyama, Masamichi ;Lother, Heinz ;Messer, Walter

• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 690 KB
• Volume: 191
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00425763

✦ Synopsis

The DNA binding protein B' preparation, isolated from the membrane of E. coli, recognizes two sites, one of which is located in the minimum oriC (35-270 bp) and the other between base pairs 417 and 488. Recognition is only possible when restriction fragments containing these sites are in single-stranded state. At the first site the strand reading 3'OH-5'P in the direction of the E. coli genetic map is recognized, at the second site the 5'P-3'OH strand.