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A revised method for the tritium labeling of pyridoxal-5′-phosphate.

✍ Scribed by O. Raibaud; M.E. Goldberg

Book ID
115904817
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
290 KB
Volume
40
Category
Article
ISSN
0014-5793

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✍ Alec Lui; Ronald Minter; Lawrence Lumeng; Ting-Kai Li 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 432 KB

Pyridoxal Y-phosphate was synthesized by first oxidizing [Wlpyridoxine to [Wlpyridoxal with MnOp ["C]Pyridoxal, isolated by cation-exchange chromatography, was then reacted with ATP in the presence of rat liver pyridoxal kinase to form ["C]pyridoxal Y-phosphate. The rat liver enzyme was used instead