๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A revised colorimetric glutamic-oxalacetic transaminase assay

โœ Scribed by Arthur L. Babson; Elsa G. Arndt; Laura J. Sharkey


Book ID
115818196
Publisher
Elsevier Science
Year
1969
Tongue
English
Weight
375 KB
Volume
26
Category
Article
ISSN
0009-8981

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๐Ÿ“œ SIMILAR VOLUMES


A new assay for glutamate-oxaloacetate t
โœ Hajime Itoh; Paul A. Srere ๐Ÿ“‚ Article ๐Ÿ“… 1970 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 294 KB

Glutamate-oxaloacetate transaminase (GOT) (n-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1) is an extensively studied enzyme (1, 2) and its content in sera from a variety of patients has important clinical diagnostic value (3). A large number of methods are available for its determination

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The action of glutamic-oxaloacetic transaminase on aspartate labeled with tritium in position 2 leads to the release of the label as tritiated water. The incubation mixture is applied to a small column of Dowex-l-OH. The labeled substrate is retained and the tritiated water is washed into a scintill