A revised colorimetric glutamic-oxalacetic transaminase assay
โ Scribed by Arthur L. Babson; Elsa G. Arndt; Laura J. Sharkey
- Book ID
- 115818196
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 375 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0009-8981
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Glutamate-oxaloacetate transaminase (GOT) (n-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1) is an extensively studied enzyme (1, 2) and its content in sera from a variety of patients has important clinical diagnostic value (3). A large number of methods are available for its determination
The action of glutamic-oxaloacetic transaminase on aspartate labeled with tritium in position 2 leads to the release of the label as tritiated water. The incubation mixture is applied to a small column of Dowex-l-OH. The labeled substrate is retained and the tritiated water is washed into a scintill