A Refined Analysis of ROS Production from Mitochondrial sn-Glycerol-3-Phosphate Dehydrogenase (mGPDH)
β Scribed by Adam L Orr; Casey L Quinlan; Irina V Perevoshchikova; Martin D Brand
- Book ID
- 113632373
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 92 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0891-5849
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An NAD-dependent glycerol-3-phosphate dehydrogenase (sn-glycerol-3-phosphate:NAD+ oxidoreductase, EC 1.1.1.8) has been isolated and purified from Saccharomyces cerevisiae by affinity and exclusion chromatography. The enzyme was purified 5 1 OO-fold to a specific activity of 158. It has a molecular w
## Abstract The mitochondrial FADβdependent glycerolβ3βphosphate dehydrogenase (FADβGPDH), recently reported in plants, has been detailed in yeast and animal systems. It oxidizes glycerolβ3βphosphate (Gβ3βP) to dihydroxyacetone phosphate (DHAP) on the outer surface of mitochondrial inner membrane.