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A real-time polymerase chain reaction assay for detection of Pneumocystis from bronchoalveolar lavage fluid

โœ Scribed by Rodney C. Arcenas; James R. Uhl; Seanne P. Buckwalter; Andrew H. Limper; Dana Crino; Glenn D. Roberts; Nancy L. Wengenack


Book ID
116407856
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
168 KB
Volume
54
Category
Article
ISSN
0732-8893

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Molecular Beacons: A Real-Time Polymeras
โœ Wilfred Chen; Grisselle Martinez; Ashok Mulchandani ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 146 KB

Molecular beacons are oligonucleotide probes that become fluorescent upon hybridization. We developed a real-time PCR assay to detect the presence of Salmonella species using these fluorogenic reporter molecules. A 122-base-pair section of the himA was used as the amplification target. Molecular bea