A new assay for 5-enolpyruvylshikimate-3-phosphate synthase is described. This enzyme of the shikimate pathway of aromatic amino acid biosynthesis generates 5-enolpyruvylshikimate 3-phosphate and orthophosphate from phosphoenolpyruvate and shikimate 3-phosphate. The shikimate pathway is present in b
โฆ LIBER โฆ
A rapid spectrophotofluorometric assay for indoleglycerol phosphate synthase
โ Scribed by Charles N. Hankins; Michael Largen; Stanley E. Mills
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 433 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
โฆ Synopsis
A rapid and sensitive spectrophotofluorometric assay for indoleglycerol phosphate synthase is presented. The method is based upon the fluorescence of the reaction product indoleglycerol phosphate and has two advantages over previously reported assays. It is more sensitive and is useful for measuring enzyme activities in extracts containing materials that prohibit the use of other methods.
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