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Rapid Assay for Nitric Oxide Synthase Using Thin-Layer Chromatography

✍ Scribed by Vijaya B. Kumar; Adonis E. Bernardo; M.M. Alshaher; M. Buddhiraju; R. Purushothaman; John E. Morley


Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
140 KB
Volume
269
Category
Article
ISSN
0003-2697

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Two novel assay systems for the determination of sialyltransferase activity using a tetramethylrhodamine-labeled disaccharide Gal␀1-4GlcNAc (2) as the acceptor are described. The TMR-labeled disaccharide 2 was synthesized by directly coupling Gal␀1-4GlcNAc-O-(CH 2 ) 6 NH 2 (1) with 5-tetramethylrhod