A rapid radioassay technique for cellular suspensions
β Scribed by John E. Byfield; Otto H. Scherbaum
- Book ID
- 102982747
- Publisher
- Elsevier Science
- Year
- 1966
- Tongue
- English
- Weight
- 544 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A simple, sensitive assay for prolylcarboxypeptidase (PCP) is described. It utilizes a radiolabeled substrate, benzyloxycarbonyl-L-prolyl-L-['H)alanine, and the details of its synthesis are also reported here. The hydrolysis of the dipeptide substrate is linear with respect to time or protein concen
## COMJIUNICATIONs muscle and kidney at pH 4.6 yields a filtrate containing all the tissue magnesium and calcium, with amounts of heavy metals too low to interfere with EDTA titration of these elements using eriochrome black T or Cal-Red as indicators.
The enzymic reduction of isotopically labeled folic acid to tetrahydrofolic acid (H,-folate) employing folic acid reductase extracted from chicken liver has been previously reported from this laboratory (1, 2). Using high specific activity tritiated folic acid (5 to 25 c/mmole) the reduction of subs