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A rapid procedure for the determination of caffeine, theophylline and theobromine in urine by micellar liquid chromatography and direct sample injection

✍ Scribed by I. Pérez-Mart́inez; S. Sagrado; M.J. Medina-Hernández

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
579 KB
Volume
304
Category
Article
ISSN
0003-2670

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✦ Synopsis

A liquid chromatographic procedure for the determination of caffeine, theophylline and theobromine in urine samples is described. The proposed system uses a Spherisorb octadecyl-silane ODS-2 C,, analytical column and a guard column of similar characteristics. The UV detector was set at 273 nm. A study to adequately select the composition of the micellar mobile phase for the separation of these compounds in urine samples is performed. Maximum resolution was achieved with a 0.075 M sodium dodecylsulphate-1.5% propanol eluent. Limits of detection at 273 nm ranged between 0.4 pg/ml for theobromine and theophylline and 1.2 pg/ml for caffeine. The procedure allows the determination of these compounds in 10 min and it can be very useful in the control of doping.

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