A rapid procedure for separation and purification of purine and pyrimidine nucleoside monophosphate kinases
โ Scribed by Maria Luisa Lombardi; Antonio Orengo
- Book ID
- 107770021
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 574 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0020-711X
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๐ SIMILAR VOLUMES
A rapid, simple, and sensitive radiochemical assay for the measurement of purine or pyrimidine nucleoside kinases (EC 2.7. I .-) is described. The substrate (thymidine, deoxyuridine, deoxycytidine, deoxyguanosine, deoxyadenosine, uridine, cytidine, and adenosine) is separated from the product (the r
Several ways of using the recently developed high-performance thin-layer chromatography (HRTLC) precoated plate NH2 F 254s to separate purines and pyrimidines are described. This precoated plate is coated with silica gel 60 which has been chemically modified with alkylamino groups. In view of the ch