β Scribed by R. Padmakumar; S. Gantla; R. Banerjee
No coin nor oath required. For personal study only.
A rapid and high-yield synthesis of methylmalonyl coenzyme (A) is reported. The two-step procedure involves preparation of the thiophenyl ester of methylmalonic acid using dicyclohexylcarbodiimide as a condensing agent, followed by transesterification with coenzyme (A), to yield methylmalonyl coenzyme (A) in (\mathbf{8 0 %}) overall yield. Inclusion of an additional step, methylation of malonic acid with iodomethane, affords the opportunity for introducing a stable or radioactive isotope into the product. This method should be applicable for the syntheses of other coenzyme (A) esters that are of biochemical interest such as succinyl coenzyme (A). c. 1993 Academic Press, Inc.
π SIMILAR VOLUMES
## It has been demonstrated that enzymes from Clostridium thermoaceticum catalyze the following reaction in which Fd is ferredoxin and CHJHF is methyltetrahydrofolate. CO2 + H2 + CH3THF + CoASH A~P,W> CH 3 COSCoA + THF + H20. The system involves hydrogenase, CO dehydrogenase, a methyltransferase