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A rapid method for the determination of glycogen content and radioactivity in small quantities of tissue or isolated hepatocytes

✍ Scribed by Timothy M. Chan; John H. Exton

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
575 KB
Volume
71
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method utilizing precipitation on filter paper has been used to isolate glycogen from homogenates of liver or isolated hepatocytes. The procedure requires very small amounts of tissue or cell suspension and is rapid and highly reproducible. Its efficiency and specificity make it very suitable for many studies involving radioactive tracer incorporation into glycogen.

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