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A rapid method for staining proteins in acrylamide gels

✍ Scribed by Gerald A. LeBlanc; Bruce J. Cochrane

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
319 KB
Volume
161
Category
Article
ISSN
0003-2697

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✦ Synopsis

A negative staining procedure for the rapid visualization of proteins in acrylamide gels is described. In the absence of proteins, staining of the gel occurs through the reduction of nitroblue tetrazolium by reduced glutathione. No staining occurs in the presence of proteins. The procedure can be completed in 20 min and is at least as sensitive as Coomassie brilliant blue Staining.

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## Mechanical stabilization of large pore acrylamide gels by addition of linear polyacrylamide Linear polyacrylamide has been used to increase the tensile strength and stability of low percentage acrylamide gels in the range of 2.5 to 6.0 % w/v acrylamide. This added strength allows handling and st