A rapid method for removal of [125I]iodide following iodination of protein solutions
โ Scribed by George P. Tuszynski; Linda Knight; Jeanette R. Piperno; Peter N. Walsh
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 574 KB
- Volume
- 106
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
โฆ Synopsis
A simple, highly effective, and rapid method for the removal of [lZSI]iodide following iodination of protein solutions is described. Greater than 98% of free iodide is removed in approximately 2 min with protein recoveries in excess of 90%. An advantage of the procedure is the confinement of radioactive waste to small easily disposable tubes.
๐ SIMILAR VOLUMES
A simple and rapid technique is described for the removal of cholate, deoxycholate and Triton X-100 from protein solutions. The procedure involves a 15min centrifugation of the sample on a Toyopearl HW40F bed prepared in a microcentrifuge tube. The merit of this method is that desalting or exchange
A protein assay is described in which the sample is precipitated with trichloroacetic acid in the presence of sodium dodecylsulfate, filtered off on a Millipore membrane and stained with Amidoschwarz 10B. The proteindye complex is eluted, and its absorbance determined at 630 nm. This assay is very r
## Abstract A quantum chemical method for rapid optimization of protein structures is proposed. In this method, a protein structure is treated as an assembly of amino acid units, and the geometry optimization of each unit is performed with taking the effect of its surrounding environment into accou