Rapid method for removal of detergents salts from protein solutions using toyopearl HW-40F

✍ Scribed by Tsuneyoshi Horigome; Hiroshi Sugano

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 553 KB
• Volume: 283
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)96267-8

✦ Synopsis

A simple and rapid technique is described for the removal of cholate, deoxycholate and Triton X-100 from protein solutions. The procedure involves a 15min centrifugation of the sample on a Toyopearl HW40F bed prepared in a microcentrifuge tube. The merit of this method is that desalting or exchange of buffer ions is possible simultaneously with the removal of detergents from protein solutions. The method is suitable for multiple assays with a high recovery of proteins and without dilution of the sample.