A Rapid Method for Extraction and Estimation of Abscisic Acid from Plant Tissue Using High Performance Liquid Chromatography
✍ Scribed by Cabot, C.; Poschenrieder, Ch.; Barceló, J.
- Book ID
- 126660372
- Publisher
- Informa UK (Taylor & Francis)
- Year
- 1986
- Tongue
- English
- Weight
- 367 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0148-3919
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A radioimmunoassay for (+)-abscisic acid (ABA) was developed and applied to the analysis of free ABA in extracts of apple (Malus pumila Mill.) and sweet pepper (Capsicum annuum L.) leaves at various stages during extract purification. Conjugates of ABA, were quantified after alkaline hydrolysis. The
Thin-layer chromatogram (6 x 6 cm) of the model mixture: (1) inorganic phosphate, (2) choline, (3) choline phosphate, (4) glycerophosphorylcholine, (5) glycerophosphate, (6) betaine. Solvent systems: First direction (vertical), methanol/water/conc. NH4 OH/chlorotorm/acetone (5 : 2 : 1 : 2 : 2); Seco