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A rapid method for determining the topological distribution of anionic sites on membrane surfaces

✍ Scribed by Nicolson, Garth L.

Publisher: Wiley (John Wiley & Sons)
Year: 1972
Tongue: English
Weight: 392 KB
Volume: 1
Category: Article
ISSN: 0091-7419
DOI: 10.1002/jss.400010209

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✦ Synopsis

A method i s described f o r the topological localization of c e l l surface anionic s i t e s using positively charged colloidal iron hydroxide (Gasic e t a l . , Lab. Invest. 18, 63 [1968]). Purified glutaraldehydc-fixed plasma c e l l membranes o r glutaraldehyde-fixed lysed c e l l s a r e mounted on thin s u p p o r t films and d i r e c t l y stained w i t h colloidal iron a t pH 1.8, washed, d r i e d , and then examined by transmission electron microscopy. Using this method the surface anionic residues on rabbit erythrocytes and murine MOPC-7OA myeloma c e l l s a r e

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