A rapid filtration assay for soluble receptors using polyethylenimine-treated filters

✍ Scribed by Robert F. Bruns; Karen Lawson-Wendling; Thomas A. Pugsley

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 688 KB
• Volume: 132
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(83)90427-x

✦ Synopsis

Ligand bound to detergent-solubilized or cytosolic receptors can be separated from free ligand by filtration through glass-fiber filters which have been pretreated with polyethylenimine (PEI). Receptors which can be assayed by this technique include detergent-solubilized muscarinic, adenosine A1, alpha 1-adrenergic, alpha 2-adrenergic, beta-adrenergic, dopamine D2, opiate, bradykinin, and benzodiazepine receptors as well as naturally soluble estradiol receptors. For muscarinic, adenosine, alpha 2, dopamine, and estradiol receptors, specific binding measured by the PEI-filter technique was 84-110% of specific binding measured by gel filtration, demonstrating that the technique gave almost quantitative recovery of bound ligand.