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A filtration assay for solubilized prolactin receptors using polyethyleneimine-treated membrane filters

✍ Scribed by Mahdy A. El-Hamzawy; Mark E. Costlow

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 377 KB
Volume: 171
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(88)90490-3

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✦ Synopsis

Free 125I-labeled ovine prolactin can be separated from detergent-solubilized prolactin-receptor complex by filtration on triacetate membrane filters pretreated with polyethyleneimine. Up to 98% of the total 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate solubilized prolactin-receptor complexes from rat liver bound to polyethyleneimine-treated membranes. This simple and rapid technique can be used to quantitate solubilized prolactin-receptor complexes.

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